Fig. 5

3DOM imaging of milk fat globule (MFG). a 3DOM (\(\rho\) component), pSIM, and WF imaging results of MFG. The white arrows indicate the solved azimuthal orientation along the surface tangent direction of the milk fat globule membrane (MFGM). b Visualization of 3DOM imaging results for MFG, with rods orientated and color-coded to indicate \(\rho\) and \(\eta\), respectively. Zoom in to show the white box region and project it in the x-z and y-z views. c Schematic representation of the structure of MFG, which contains a triacylglycerol (TAG) core and a triple-layer structure. KK114 dye labels the phospholipid on the MFGM. GP, glycerophospholipids; SM, sphingomyelin; CHOL, cholesterol. d The experimental deviation curve for \(\rho\) in (b) is estimated based on the MFGM tangent direction. e, f Imaging and the statistics distribution of \(\eta\) orientation within z-slice with spacing steps at 1 μm. Scale bars (a, b, e), 2 μm; (b1-b3), 200 nm