Three-step one-way model in terahertz biomedical detection

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Table 1 Experimental process of alkaline hydrolysis (AH)

	Blank tube	Standard tube	Sample tube
Distilled water (mL)	1.0
5 μg/mL standard working solution (mL)		1.0
Supernatant (mL)			1.0
Dichloramine-T (mL)	0.5	0.5	0.5
Mix sufficiently and let stand for 10 min.
Perchloric acid (mL)	0.5	0.5	0.5
Mix sufficiently and let stand for 10 min.
Dimethylaminobenzaldehyde (mL)	0.5	0.5	0.5

The reagents were mixed well, and the mixture was placed in a 60 °C water bath for 15 min, cooled to room temperature, and centrifuged at 3500 rpm for 10 min. The supernatant was taken and transferred into the spectrometer (at 550 nm) which is from Thermo labsystem 353 to measure the absorption (OD values) of all tubes (distilled water was used as a blank)