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Fig. 4 | PhotoniX

Fig. 4

From: Multi-focus light-field microscopy for high-speed large-volume imaging

Fig. 4

Volumetric imaging of vascular structures of zebrafish larvae. a Sub-aperture components rearranged from sLFM and SAsLFM images are shown for comparison. The central components (u = (8, 8)) of these two methods are focused at the same depth for fair comparison and exhibit equal sharpness. Other angular components of SAsLFM (u = (8, 4)) and (u = (6, 11)) show finer detail than those of sLFM. The estimated phases at the pupil planes of sLFM and SAsLFM are inserted in the figures. b Intensity profiles along the yellow lines in a. c Sharpness versus sub-aperture index on the data captured by sLFM and SAsLFM. d 3D rendered volume obtained by SAsLFM. e Magnified views of yellow boxed regions in d, indicating that SAsLFM provides significant improvement with eliminated artifacts (marked with the white arrows) and higher resolution (marked with the yellow arrows). f The reconstructed slices of SAsLFM provide higher resolution at various depths, which is highlighted by the yellow arrows. Scale bars: 500 μm (a, d) and 150 μm (f)

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