Fig. 7

AO improves in vivo structural and functional imaging in the hypothalamus of a zebrafish larva. a Maximum intensity projection of the neuronal structures 262–267 μm below the skin without and with AO correction. b Maximum intensity projection of the neuronal structures axially centered 274 μm below the skin, which were used for aberration measurement. c Corrective wavefront. The dashed squares indicate the layout of the wavefront segments, where each wavefront segment corresponds to a unit square. A 2 × 2 with 1 × 1 stepped overlapping mask approach was used in the measurement process. d Zoomed-in views of the orange boxes. e Intensity profiles along the pink lines in (d). f Maximum intensity projection over time of a plane 279 μm below the skin without and with AO correction. g Fluorescence signals during calcium imaging at four regions of interest (ROIs) without and with AO correction. The ROIs are marked by cyan arrows in (f). Scale bars, 10 μm in (a), (b), and (f) and 5 μm in (d)