Fig. 4

a DIC and fluorescence images from time series showing changes in actin distribution within cells exposed to a single 5 kV/cm nsPEF with yellow arrows pointing toward areas of membrane bound cortical actin and intracellular diffuse actin fluorescence. b Average change in relative fluorescence (∆F/F_o) measurements from cells within the field of view for the above time series images. Intracellular measurements were recorded with traces inside the cell membrane, whole cell measurements were recorded from traces outside the cell membrane, and membrane specific measurements were recorded from inside of the annulus of the previous two traces