Fig. 1
From: Brillouin microscopy monitors rapid responses in subcellular compartments
a (top) Conceptual illustration with examples of stimuli and cell behaviors linked to changes in cellular mechanics, (middle) cell responses, such as filament depolymerization, membrane disruption, and channel activation, and (bottom) examples of microscopic imaging modalities used in this report. Instrument capabilities and measurements include: b DIC imaging, indicators color-matched to c providing examples of locations used for Brillouin measurements taken from the subcellular compartments. c Average Brillouin frequency shift measurements from each of the three compartments. d Representative example of Brillouin and Raman spectral measurements from within a cell nucleolus. e Example of 3D mechanical map from CHO-K1 cell via z-stack of 2D Brillouin images. a Created with BioRender.com.