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Fig. 6 | PhotoniX

Fig. 6

From: Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging

Fig. 6

Long-term volumetric super-resolution imaging of live cells with adaptively trained PRS-SIM. a 3D-SIM imaging of a live COS7 cell expressing 3xmEmerald-Ensconsin (green) and Lamp1-Halo (red) (Supplementary Video 3). The WF, Conv. 3D-SIM, and PRS-SIM results are compared. Scale bar, 2 μm. b, c Time-lapse PRS-SIM images of the dynamic interaction between lysosomes (Lyso) and microtubules (MTs) as the Lyso is moving along adjacent MTs (b) or deforming under the traction of MTs (c). Scale bar, 1 μm. d PRS-SIM enhanced LLS-SIM images of a live COS7 cell expressing TOMM20-2xmEmerald (magenta) and 3xmCherry-Ensconsin (green) (Supplementary Video 4). The zoom-in comparison of WF, Conv. SIM and PRS-SIM are displayed in the corner. Scale bar, 5 μm (regular), 1 μm (zoom-in). e, f Time-lapse recording of the rapid fission (e) and fusion (f) processes of mitochondria (Mito) interacted with MTs. The denoised images of N2N-SIM and PRS-SIM are compared. Scale bar, 1 μm

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