Fig. 4

PRS-SIM for multimodal SIM systems. a 3D-SIM images of lysosomes in fixed COS7 cells reconstructed with Conv. SIM and PRS-SIM accompanied with the corresponding GT-SIM image. Single slice view of the square region are provided for visualizing the details. Scale bar: 2 μm (regular), 0.5 μm (zoom-in regions). b Intensity profiles of Conv. SIM (blue), Sparse-SIM (green), PRS-SIM (red), and GT-SIM (brown) along the line indicated by the arrowheads in a. c LLS-SIM images of mitochondria in fixed COS7 cells reconstructed with Conv. SIM and PRS-SIM. Scale bar, 2 μm. d Representative single-slice image of the squared region in c. Scale bar, 1 μm. e NL-SIM images of F-actin in COS7 cells. WF, PRS-SIM and GT-SIM images are shown. f The FRC curves of the samples in e. The resolution is calculated based on the cutoff frequency with a threshold of 0.24. Scale bar, 5 μm (regular), 1 μm (zoom-in). In a and c, the XY plane is displayed in maximum intensity projection (MIP) view and the XZ plane is displayed in sectioned view (indicated by white dashed lines)