Skip to main content
PhotoniX
Fig. 2 | PhotoniX

Fig. 2

From: Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging

Fig. 2

Fidelity and resolution evaluation of PRS-SIM. a TIRF-SIM images of clathrin coated pits (CCPs), microtubules (MTs), and endoplasmic reticulum (ER) reconstructed and processed with Conv. SIM, Sparse-SIM, and PRS-SIM. Corresponding WF and GT-SIM images are provided for reference. Scale bar, 1 μm. b Fourier spectra of WF, PRS-SIM and, GT-SIM images of a MT sample. The dashed circle denotes the cutoff frequency (corresponding to the spatial frequency of 94 nm). c Quantitative comparison among PRS-SIM, Conv. SIM and Sparse-SIM. The PSNR and SSIM values are calculated referring to GT-SIM images (N = 40 for each data point). d Intensity profiles of Conv. SIM (blue), Sparse-SIM (green), PRS-SIM (red), and GT-SIM (brown) along the line indicated by the yellow arrowheads in a. e Fourier ring correlation curves of the WF, PRS-SIM and GT-SIM images in b. The resolution is calculated according to the cutoff frequency with an FRC threshold of 0.24

Back to article page