Fig. 4

Time-lapse AO 2P-MSIM imaging of motoneurons extended from the spinal cord. Time-lapse AO 2P-MSIM imaging of mnx1:mGFP zebrafish motoneurons extended from the spinal cord at 210-230-μm depth. A Representative MIPs of the Sys AO WF, Full AO WF, and Full AO MSIM images. Arrows point to highly dynamic structures. Scale bar, 5 μm. B Lateral resolution improvement by AO correction and MSIM reconstruction is quantified in spatial frequency space calculated from images of the motoneurons at 218-μm depth (0 min). C Higher magnification views of time-lapse AO off WF and AO on MSIM images for the region pointed by purple arrows in (A). Scale bar, 2 μm