Fig. 3

AO 2P-MSIM imaging of dense motoneurons in living mnx1:mGFP zebrafish. In vivo AO 2P-MSIM imaging of dense mnx1:mGFP zebrafish motoneurons in the spinal cord. A Sys AO WF, Full AO WF, and Full AO MSIM images in the same field of view. The signal from the Sys AO WF image was artificially increased (2-fold) for better visualization. B Higher magnification views of the top boxed region in (A) are shown. C Line profiles across an axonal structure corresponding to (B). D Higher magnification views of the bottom boxed region in (A) are shown. White arrows indicate a ring structure revealed with significant resolution improvement after sample-induced AO correction and MSIM reconstruction. E Wavefronts before and after sample correction are shown. Scale bar, 2 μm