Fig. 2

AO 2P-MSIM improves spatial resolution in a brain slice. AO 2P-MSIM imaging in a Thy1-YFP brain slice at 325-313-μm depth. A Maximum intensity projection (MIP) of full AO WF and full AO MSIM image stacks (1-μm Z step). B Higher magnification views of the top boxed region in (A) at 316-μm depth are shown. Sys AO WF and Full AO WF indicate widefield images without and with sample AO correction, respectively, and Full AO MSIM indicates the MSIM reconstructed image with sample AO correction. White arrows indicate obvious resolution improvement. C Lateral resolution improvement after AO correction and MSIM reconstruction is quantified in spatial frequency space. D Higher magnification views of the bottom boxed region in (A) at 321 μm and 322 μm are shown. White arrows indicate the dendritic spines. E Line profiles across the dendritic spine neck corresponding to (D). The signal was normalized to the AO condition (0 to the maximum intensity of the Full AO WF image). F Wavefronts detected by SHS before and after sample AO correction are shown. Scale bar, 2 μm