Fig. 1

Schematic diagram of AO 2P-MSIM system. Schematic diagram of AO 2P-MSIM system. 1: SLM; 2: objective; 3: DM; 4: SHS; 5: EMCCD. The flip mirror can be switched between the EMCCD and SHS. L1–L9: lenses. F1 and F2: filters. Purple rectangle: Wavefront detection. The SLM loads phase pattern for a single focus to exciting specimen; The SHS detects aberration wavefront, while the DM is set to a flat mirror. Green rectangle: Super-resolution imaging with AO correction. The SLM loads phase patterns for an AO-corrected laser focus array; The DM loads corrective voltages to compensate for the fluorescence aberrations; The EMCCD captures corrected images. Scale bar, 5 μm