Table 2 Examples of primary antibodies for immuno-fluorescence staining with reported binding specificity and features examples for digital staining (DS). MAP2 = Microtubule Associated Protein 2, HEK = human embryonic kidney, isoform specificity = “no detectable non-specific binding”
From: Digital staining in optical microscopy using deep learning - a review
Antibody | Target molecule | Reported binding specificity | Featured DS references |
|---|---|---|---|
Anti-\(\beta\)-actin | cytoskeleton (actin proteins) | isoform specificity in knockout cells [96] | |
Anti-MAP2 | dendrites (microtubule assembly during neurogenesis) | 90% in Mouse brain cells [97] | |
Anti-Tau | axons (neuronal microtubule-associated protein) | 80% for pT181 binding site isoform specificity for most other sites in HEK cells [99] | |
Anti-Ki-67 | cell cycle marker, proliferation marker, marker for neoplastic tissues | 66% in human blood cells [100] |