Table 1 Most common fluorophores for natural autofluorescence, according to [49]
From: Digital staining in optical microscopy using deep learning - a review
Fluorophore | Excitation wavelength (nm) | Emission wavelength (nm) |
|---|---|---|
Phe, Tyr, Trp | 240 - 280 | 280 - 350 |
Cytokeratins | 280 - 325 | 495 - 525 |
Collagen | 330 - 340 | 400 - 410 |
Elastin | 350 - 420 | 420 - 510 |
NAD(P)H | 330 - 380 | 440 - 462 |
Flavins | 350 - 370, 440 - 450 | 480, 540 |
Fatty acids | 330 - 350 | 470 - 480 |
Vitamin A | 370 - 380 | 490 - 510 |
Porphyrins | 405 | 630 - 700 |
Lipofuscins | 400 - 500 | 480 - 700 |